Pratelli Annamaria, Moschidou Paschalina
Department of Public Health and Animal Sciences, Strada per Casamassima km 3, 70010 Valenzano, Bari, Italy.
J Vet Diagn Invest. 2012 Sep;24(5):981-5. doi: 10.1177/1040638712453579. Epub 2012 Jul 23.
Canine minute virus (CnMV) is a member of the Bocavirus genus, together with Bovine parvovirus (BPV), which emerged as a new pathogen of dogs in 1967. The genus Bocavirus gained more recognition after the recent identification of Human bocavirus in pooled specimens from the respiratory tract of children. The cell culture host range of CnMV appears to be restricted to the Walter Reed canine cell (WRCC) line, although there is a report that indicates susceptibility of the Madin-Darby canine kidney (MDCK) cell line. In order to study the susceptibility of different cell lines to CnMV, the replication in cell cultures of canine, bovine, and human origin and the interaction of the virus with freshly isolated canine peripheral blood mononuclear cells were evaluated. The in vitro host range was unexpectedly wide. As shown by indirect fluorescent antibody and polymerase chain reaction assays, CnMV replicates efficiently in the A72 and MDCK canine cell lines. Bovine and human cell lines support the replication of CnMV less efficiently. In contrast, canine mononuclear blood cells are permissive to replication of CnMV in vitro. The present study revealed the ability of CnMV to replicate in continuous cell lines of different origin and, partially, in canine mononuclear cells.
犬微小病毒（CnMV）是博卡病毒属的成员之一，与牛细小病毒（BPV）同属该属，它于1967年作为犬类的一种新病原体出现。在最近从儿童呼吸道混合标本中鉴定出人博卡病毒后，博卡病毒属得到了更多的关注。CnMV的细胞培养宿主范围似乎仅限于沃尔特·里德犬细胞（WRCC）系，不过有一份报告表明，马-达二氏犬肾（MDCK）细胞系对其敏感。为了研究不同细胞系对CnMV的敏感性，对犬源、牛源和人源细胞培养物中的病毒复制情况以及该病毒与新分离的犬外周血单核细胞的相互作用进行了评估。其体外宿主范围出人意料地广泛。间接荧光抗体和聚合酶链反应分析表明，CnMV能在A72和MDCK犬细胞系中高效复制。牛和人细胞系对CnMV复制的支持效率较低。相比之下，犬单核血细胞在体外允许CnMV复制。本研究揭示了CnMV在不同来源的连续细胞系以及部分犬单核细胞中复制的能力。
