Department of Biotechnology, Lovely Professional University, Phagwara, Punjab, India.
Cell Biochem Biophys. 2013 May;66(1):45-51. doi: 10.1007/s12013-012-9396-4.
Proteases are of particular interest because of their action on insoluble keratin substrates and generally on a broad range of protein substrates. Proteases are one of the most important groups of industrial enzymes used in detergent, protein, brewing, meat, photographic, leather, dairy, pharmaceutical and food industry. In the present study, the organism isolated from the protein rich soil sample was identified by biochemical and molecular characterisation as Bacillus thuringiensis and further optimum conditions for alkaline protease synthesis were determined. The growth conditions for B. thuringiensis was optimised by inoculating into yeast extract casein medium at different pH and incubating at different temperatures. The maximum protease production occurred at pH 8 and at 37 °C. B. thuringiensis showed proteolytic activity at various culture conditions. Optimum conditions for the protease activity were found to be 47 °C and pH 8. In the later stage, the blood removing action of crude and partially purified protease was found to be effective within 25 min in the presence of commercial detergents indicating the possible use of this enzyme in detergent industry. Enzyme also showed good activity against hair substrate keratin and can be used for dehairing.
蛋白酶因其对不溶性角蛋白底物的作用以及通常对广泛的蛋白质底物的作用而特别受到关注。蛋白酶是用于洗涤剂、蛋白质、酿造、肉类、摄影、皮革、乳制品、制药和食品工业的最重要的工业酶类之一。在本研究中，从富含蛋白质的土壤样品中分离出的微生物通过生化和分子特征鉴定为苏云金芽孢杆菌，并进一步确定了碱性蛋白酶合成的最佳条件。通过在不同 pH 值下接种酵母提取物酪蛋白培养基并在不同温度下孵育，优化了苏云金芽孢杆菌的生长条件。在 pH 8 和 37°C 时，蛋白酶的产量最高。苏云金芽孢杆菌在各种培养条件下均表现出蛋白水解活性。发现蛋白酶活性的最佳条件为 47°C 和 pH 8。在后期阶段，在商业洗涤剂存在的情况下，粗酶和部分纯化酶的除血作用在 25 分钟内有效，表明该酶在洗涤剂工业中有潜在用途。该酶对毛发基质角蛋白也表现出良好的活性，可用于脱毛。
