Lu Y, Mumaw J L, West F D, Stice S L
Department of Animal and Dairy Science, Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA.
Reprod Domest Anim. 2012 Aug;47 Suppl 4:72-6. doi: 10.1111/j.1439-0531.2012.02057.x.
Chimeric animals generated from livestock-induced pluripotent stem cells (iPSCs) have opened the door of opportunity to genetically manipulate species for the production of biomedical models, improving traits of agricultural importance and potentially providing a system to test novel iPSC therapies. The potential of pluripotent stem cells in livestock has long been recognized, with many attempts being chronicled to isolate, culture and characterize pluripotent cells from embryos. However, in most cases, livestock stem cells derived from embryonic sources have failed to reach a pluripotent state marked by the inability to form chimeric animals. The in-depth understanding of core pluripotency factors and the realization of how these factors can be harnessed to reprogram adult cells into an induced pluripotent state has changed the paradigm of livestock stem cells. In this review, we will examine the advancements in iPSC technology in mammalian and avian livestock species.
由家畜诱导多能干细胞（iPSC）产生的嵌合体动物为通过基因操作物种来生产生物医学模型、改善具有农业重要性的性状以及潜在地提供一个测试新型iPSC疗法的系统打开了机遇之门。家畜中多能干细胞的潜力早已得到认可，人们进行了许多尝试来分离、培养和鉴定胚胎来源的多能细胞。然而，在大多数情况下，源自胚胎的家畜干细胞未能达到以无法形成嵌合体动物为标志的多能状态。对核心多能性因子的深入理解以及认识到如何利用这些因子将成体细胞重编程为诱导多能状态，改变了家畜干细胞的范式。在这篇综述中，我们将探讨哺乳动物和禽类家畜物种中iPSC技术的进展。
