Rheumatology Division, CIBER-BBN/ISCIII, Tissue Engineering and Cellular Therapy Group (CBTTC-CHUAC), INIBIC-Hospital Universitario A Coruña, A Coruña, Spain.
J Biomed Mater Res A. 2013 Jan;101(1):157-66. doi: 10.1002/jbm.a.34312. Epub 2012 Jul 25.
Sensitive hydrogels attract interest due to their soft wet appearance and shape response to environmental variations. The synthesis and characterization of semi-interpenetrated hydrogels obtained by radical-induced polymerization of 2-ethyl-(2-pyrrolidone)methacrylate (EPM) in the presence of different concentrations of hyaluronic acid (HA) using N,N'-methylene-bisacrylamide or triethylene glycol dimethacrylate as crosslinker, followed by freeze-drying, are described. Polymeric systems were characterized by NMR, FTIR, SEM, TGA, and DMA. PEPMHA hydrogels' mechanical properties and swelling were found to be intimately related to HA concentration and crosslinker. The swelling response was assessed for temperature and pH variation in order to study the behavior of the hydrogels. We found that the presence of HA in PEPM polymeric systems induced a sensitivity to pH variation rather than temperature. Finally, the biocompatibility profile of the hydrogels was evaluated, using mesenchymal stem cells. Cell adhesion and proliferation results revealed the non-cytotoxicity of the systems. We estimate that PEPMHA hydrogels can be used for applications in tissue engineering and for the controlled release of bioactive compounds.
由于其柔软湿润的外观和对环境变化的形状响应，敏感水凝胶引起了人们的兴趣。本文描述了在不同浓度透明质酸（HA）存在下，通过自由基引发 2-乙基-（2-吡咯烷酮）甲基丙烯酸酯（EPM）聚合，使用 N，N'-亚甲基双丙烯酰胺或三乙二醇二甲基丙烯酸酯作为交联剂，随后进行冷冻干燥，合成和表征半互穿水凝胶。通过 NMR、FTIR、SEM、TGA 和 DMA 对聚合体系进行了表征。PEPMHA 水凝胶的机械性能和溶胀性能与 HA 浓度和交联剂密切相关。为了研究水凝胶的行为，评估了其对温度和 pH 值变化的溶胀响应。我们发现，HA 的存在诱导了 PEPM 聚合物体系对 pH 值变化而不是温度的敏感性。最后，使用间充质干细胞评估了水凝胶的生物相容性。细胞黏附和增殖结果表明该体系无细胞毒性。我们估计，PEPMHA 水凝胶可用于组织工程和生物活性化合物的控制释放。
