Shaĭkevich E V, Ivshina E V, Zakharov I A
Genetika. 2012 May;48(5):666-71.
In geographically distant populations of ladybird beetle Adalia bipunctata from Eurasia mitotypes and infection with symbiotic bacteria Spiroplasma and Rickettsia were determined. All populations examined demonstrated mtDNA polymorphism and striking differences in prevalence of bacteria (from about 50% of individuals infected with Spiroplasma in St.-Petersburg population and 50% of the Rickettsia prevalence in Kem' population to complete absence of bacteria in the population from Archangelsk). In the populations studied a total of 14 mitotypes were discovered, including two mitotypes that were remarkably different from the others in nucleotide composition. Mitotype 10, which was the most different from all the others, was found in all populations from Germany to Transbaikalia, excluding the population from Tashkent. Linkage disequilibrium between mitotype 10 and the Rickettsia infection was confirmed. Infection with the Spiroplasma bacteria was typical of the individuals with haplotype 1 and relative to it. The results obtained supported the conclusion on the association between infection with Spiroplasma and Rickettsia and certain mitotype of A. bipunctata, which was the consequence of either absence or rare horizontal transfer of symbionts and ancientness of the first contact between the bacteria and A. bipunctata ladybird beetles.
在来自欧亚大陆的二斑叶螨地理上相距遥远的种群中，确定了线粒体类型以及共生细菌螺旋体属和立克次氏体的感染情况。所有检测的种群都表现出线粒体DNA多态性以及细菌感染率的显著差异（从圣彼得堡种群中约50%的个体感染螺旋体属，科姆种群中立克次氏体感染率为50%，到阿尔汉格尔斯克种群中完全没有细菌）。在所研究的种群中总共发现了14种线粒体类型，其中包括两种在核苷酸组成上与其他类型明显不同的线粒体类型。线粒体类型10与其他所有类型差异最大，在从德国到外贝加尔的所有种群中都有发现，但不包括塔什干的种群。线粒体类型10与立克次氏体感染之间的连锁不平衡得到了证实。感染螺旋体属细菌是单倍型1个体及其相关个体的典型特征。所获得的结果支持了关于螺旋体属和立克次氏体感染与二斑叶螨特定线粒体类型之间存在关联的结论，这是共生体缺乏或罕见水平转移以及细菌与二斑叶螨首次接触古老性的结果。
