Potier S, Lacroute F, Hubert J C, Souciet J L
Institut de Biologie Moléculaire et Cellulaire C.N.R.S., Strasbourg, France.
FEMS Microbiol Lett. 1990 Oct;60(1-2):215-9. doi: 10.1016/0378-1097(90)90374-y.
The multifunctional protein carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase) encoded by the URA2 gene catalyses the first two steps of the yeast pyrimidine pathway. An excess of the final product, the intracellular UTP (uridine triphosphate), inhibits both the transcription of the URA2 gene and the enzymatic activities. Results presented in this paper suggest that transcription of URA2 is negatively regulated (repression-derepression) and establish that this regulation is less efficient in the flow of the pyrimidine pathway than feedback inhibition.
URA2基因编码的多功能蛋白氨甲酰磷酸合成酶（CPSase）-天冬氨酸转氨甲酰酶（ATCase）催化酵母嘧啶途径的前两个步骤。过量的终产物，即细胞内的三磷酸尿苷（UTP），会抑制URA2基因的转录和酶活性。本文给出的结果表明，URA2的转录受到负调控（阻遏-去阻遏），并证实这种调控在嘧啶途径的流程中不如反馈抑制有效。
