哺乳动物大脑中的内源性类洋地黄活性。
Endogenous digitalis-like activity in mammalian brain.
作者信息
Fishman M C
出版信息
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4661-3. doi: 10.1073/pnas.76.9.4661.
Abstract
A fraction of brain has been prepared that contains a substance mimicking actions of the digitalis glycosides. It both blocks the binding of [3H]ouabain to Na+,K+-ATPase (ATP phosphohydrolase, EC 3.6.1.3) and inhibits the uptake of 86Rb+ into human erythrocytes. Partial isolation was achieved by Sephadex G-10 fractionation and subsequent desalting. This activity is potentially capable of regulating ionic fluxes and concentration gradients across the cell membrane.
摘要
已制备出一部分大脑组织，其中含有一种模拟洋地黄苷作用的物质。它既能阻断[3H]哇巴因与Na +，K + -ATP酶（ATP磷酸水解酶，EC 3.6.1.3）的结合，又能抑制86Rb +进入人红细胞。通过Sephadex G - 10分级分离和随后的脱盐实现了部分分离。这种活性可能能够调节跨细胞膜的离子通量和浓度梯度。
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