Taneja M, Pareek P K, Jatkar P R
Department of Obstetrics and Gynaecology, College of Veterinary and Animal Science, Rajasthan Agricultural University, Bikaner, India.
Indian J Exp Biol. 1990 Nov;28(11):1031-3.
Present investigation was conducted to study the ovarian response and embryo recovery using different PMSG dose levels. Six rabbits each were assigned randomly to treatment 1 (PMSG 50 IU + hCG 100 IU), treatment 2 (PMSG 75 IU + hCG 100 IU) and the control group (no hormone administered). PMSG injection (im) was followed 68 hr later by natural mating to a fertile rabbit buck and human chorionic gonadotrophin (hCG) injection iv post coitum. Embryos were recovered 96 hr post coitum by a modified surgical method. Mean number of ovulations in the control group differed significantly from treatment 1 and 2, but no significant difference was observed between treatments 1 and 2. Mean embryo recovery percentage was lowest in treatment 2 and highest in the control group. The higher dose PMSG (treatment 2) was observed to be more disturbing in terms of recovery of embryos as well as their morphology.
本研究旨在使用不同剂量的孕马血清促性腺激素(PMSG)来研究卵巢反应和胚胎回收情况。将18只兔子随机分为3组,每组6只。第1组注射50国际单位PMSG + 100国际单位人绒毛膜促性腺激素(hCG);第2组注射75国际单位PMSG + 100国际单位hCG;对照组不注射任何激素。肌肉注射PMSG,68小时后与可育雄兔自然交配,交配后静脉注射hCG。交配96小时后,通过改良手术方法回收胚胎。对照组的平均排卵数与第1组和第2组有显著差异,但第1组和第2组之间无显著差异。胚胎回收的平均百分比在第2组中最低,在对照组中最高。观察到较高剂量的PMSG(第2组)在胚胎回收及其形态方面更具干扰性。