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使用不同剂量孕马血清促性腺激素(PMSG)对家兔(穴兔)进行超数排卵和胚胎回收

Superovulation and embryo recovery in rabbits (Oryctolagus cuniculus) using different doses of pregnant mare serum gonadotrophin (PMSG).

作者信息

Taneja M, Pareek P K, Jatkar P R

机构信息

Department of Obstetrics and Gynaecology, College of Veterinary and Animal Science, Rajasthan Agricultural University, Bikaner, India.

出版信息

Indian J Exp Biol. 1990 Nov;28(11):1031-3.

PMID:2283171
Abstract

Present investigation was conducted to study the ovarian response and embryo recovery using different PMSG dose levels. Six rabbits each were assigned randomly to treatment 1 (PMSG 50 IU + hCG 100 IU), treatment 2 (PMSG 75 IU + hCG 100 IU) and the control group (no hormone administered). PMSG injection (im) was followed 68 hr later by natural mating to a fertile rabbit buck and human chorionic gonadotrophin (hCG) injection iv post coitum. Embryos were recovered 96 hr post coitum by a modified surgical method. Mean number of ovulations in the control group differed significantly from treatment 1 and 2, but no significant difference was observed between treatments 1 and 2. Mean embryo recovery percentage was lowest in treatment 2 and highest in the control group. The higher dose PMSG (treatment 2) was observed to be more disturbing in terms of recovery of embryos as well as their morphology.

摘要

本研究旨在使用不同剂量的孕马血清促性腺激素(PMSG)来研究卵巢反应和胚胎回收情况。将18只兔子随机分为3组,每组6只。第1组注射50国际单位PMSG + 100国际单位人绒毛膜促性腺激素(hCG);第2组注射75国际单位PMSG + 100国际单位hCG;对照组不注射任何激素。肌肉注射PMSG,68小时后与可育雄兔自然交配,交配后静脉注射hCG。交配96小时后,通过改良手术方法回收胚胎。对照组的平均排卵数与第1组和第2组有显著差异,但第1组和第2组之间无显著差异。胚胎回收的平均百分比在第2组中最低,在对照组中最高。观察到较高剂量的PMSG(第2组)在胚胎回收及其形态方面更具干扰性。

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