Dept. of Food Technology, University Miguel Hernández, Ctra. Beniel km. 3.2, 03312, Orihuela, Alicante, Spain.
Int J Food Microbiol. 2012 Aug 17;158(2):101-6. doi: 10.1016/j.ijfoodmicro.2012.07.002. Epub 2012 Jul 10.
In this work the antifungal efficacy of thymol, carvacrol and the mixture of both pure essential oils has been proved against Penicillium digitatum and Penicillium italicum, by using in vitro (liquid media, PDA plates and disk growth) and in vivo (lemon) tests. Results indicated that both essential oils were effective in inhibiting fungal growth in all in vitro tests, with the highest efficacy displayed by thymol. In addition, the application of wax with thymol and carvacrol to lemons inoculated with P. digitatum demonstrated the reduction of decay (expressed as percentage of infected fruit surface) in a concentration dependent manner, as well as reduced respiration rate, ethylene production and total acidity losses. Thus, the application of these essential oils together with wax in the citrus packing lines could be considered as good alternatives to reduce the use of synthetic fungicides.
在这项工作中，通过体外（液体培养基、PDA 平板和圆盘生长）和体内（柠檬）试验，证明了百里香酚、香芹酚和两者混合的纯精油对桔青霉和意大利青霉具有抗真菌功效。结果表明，两种精油在所有体外试验中都能有效抑制真菌生长，其中百里香酚的效果最佳。此外，将含有百里香酚和香芹酚的蜡应用于接种桔青霉的柠檬上，证明了腐烂（用感染果实表面的百分比表示）的减少与浓度呈依赖性，同时还降低了呼吸速率、乙烯生成和总酸度损失。因此，在柑橘包装线上应用这些精油和蜡可以被认为是减少合成杀菌剂使用的良好替代品。
