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下一代食品发酵微生物生态学方法。

Next-generation approaches to the microbial ecology of food fermentations.

机构信息

Department of Viticulture and Enology, University of California, Davis, CA 95616, USA.

出版信息

BMB Rep. 2012 Jul;45(7):377-89. doi: 10.5483/bmbrep.2012.45.7.148.

Abstract

Food fermentations have enhanced human health since the dawn of time and remain a prevalent means of food processing and preservation. Due to their cultural and nutritional importance, many of these foods have been studied in detail using molecular tools, leading to enhancements in quality and safety. Furthermore, recent advances in high-throughput sequencing technology are revolutionizing the study of food microbial ecology, deepening insight into complex fermentation systems. This review provides insight into novel applications of select molecular techniques, particularly next-generation sequencing technology, for analysis of microbial communities in fermented foods. We present a guideline for integrated molecular analysis of food microbial ecology and a starting point for implementing next-generation analysis of food systems.

摘要

食品发酵自远古时代以来就增进了人类健康,并且仍然是一种流行的食品加工和保存方式。由于其文化和营养价值,许多这些食品已经使用分子工具进行了详细研究,从而提高了质量和安全性。此外,高通量测序技术的最新进展正在彻底改变食品微生物生态学的研究,加深了对复杂发酵系统的了解。本综述提供了有关特定分子技术(特别是下一代测序技术)在发酵食品中微生物群落分析中的新颖应用的深入了解。我们提出了食品微生物生态学综合分子分析的指南,并为实施下一代食品系统分析提供了起点。

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