Developmental Neuroscience, Munroe Meyer Institute, University of Nebraska Medical Center, 985960 Nebraska Medical Center, Omaha, NE 68918, USA.
Microsc Microanal. 2012 Aug;18(4):742-4. doi: 10.1017/S1431927612000256. Epub 2012 Jul 26.
Advances in microscopy allow one to probe the structure of neurons and their interactions with astrocytes in brain slices and in vivo at ever increasing resolution. Moreover, the dynamic interactions between the cells can be examined in live preparation. In this paper we discuss how a variety of imaging approaches: confocal microscopy, electron microscopy, and multiphoton time-lapse microscopy are employed to probe neuron glia interactions in the developing cerebellum.
显微镜技术的进步使得人们能够以越来越高的分辨率在脑切片和体内探测神经元的结构及其与星形胶质细胞的相互作用。此外，还可以在活体标本中研究细胞之间的动态相互作用。本文讨论了各种成像方法（共聚焦显微镜、电子显微镜和多光子时差显微镜）如何用于探测发育中小脑的神经元-神经胶质相互作用。
