Department of Pathology, Yonsei University, Wonju College of Medicine, Wonju, South Korea.
Pathobiology. 2013;80(1):41-52. doi: 10.1159/000339513. Epub 2012 Jul 24.
The purpose of this study was to investigate the expression of metabolism-related proteins such as Glut-1 and carbonic anhydrase IX (CAIX) according to breast cancer molecular subtype.
We generated a tissue microarray of 276 breast cancer patients and performed immunohistochemical staining for known metabolism-related proteins, which were evaluated according to the molecular subtype.
The expression of IGF-1, MIF, and HIF-1α was correlated with the HER-2 type (p < 0.05). Glut-1 overexpression and CAIX expression were associated with TNBC type, especially with basal-like type, high histologic grade, estrogen receptor negativity, and progesterone receptor negativity (p < 0.05). The expression of Glut-1 and CAIX was correlated with statistical significance (p < 0.001).
We identified different patterns of expression of metabolism-related proteins according to the molecular subtypes of breast cancer defined by surrogate immunohistochemistry. Increased expression of HIF-1α, IGF-1, and MIF was noted in HER-2 type breast cancer and increased expression of Glut-1 and CAIX was noted in TNBC type breast cancer, especially in the basal-like subtype, which exhibited a glycolytic and acid-resistant tumor phenotype.
本研究旨在探讨根据乳腺癌分子亚型表达代谢相关蛋白，如 Glut-1 和碳酸酐酶 IX（CAIX）。
我们生成了 276 例乳腺癌患者的组织微阵列，并对已知的代谢相关蛋白进行了免疫组织化学染色，根据分子亚型进行了评估。
IGF-1、MIF 和 HIF-1α 的表达与 HER-2 型相关（p<0.05）。Glut-1 过表达和 CAIX 表达与三阴性乳腺癌（TNBC）类型相关，特别是与基底样型、高组织学分级、雌激素受体阴性和孕激素受体阴性相关（p<0.05）。Glut-1 和 CAIX 的表达与统计学显著相关（p<0.001）。
我们根据免疫组织化学替代物定义的乳腺癌分子亚型，确定了代谢相关蛋白表达的不同模式。在 HER-2 型乳腺癌中观察到 HIF-1α、IGF-1 和 MIF 的表达增加，在 TNBC 型乳腺癌中观察到 Glut-1 和 CAIX 的表达增加，特别是在基底样型中，表现出糖酵解和耐酸性肿瘤表型。
