Shofstahl J H, Hardy J K
Millipore Corporation, Waters Chromatography Division, Milford, Massachusetts 01757.
J Chromatogr Sci. 1990 May;28(5):225-9. doi: 10.1093/chromsci/28.5.225.
The United States Environmental Protection Agency (EPA) currently specifies atomic absorption spectrophotometry as the method of analysis for the priority pollutant metals. While the method is very sensitive, it does suffer from limitations. As an alternative, the use of dibenzyldithiocarbamate (DBDC) to complex the priority pollutant metals from the sample, with subsequent separation of the complexes by liquid chromatography and quantification by ultraviolet-visible spectrophotometry, is reported. Of the thirteen priority pollutant metals, ten can be determined with the method (Cd, Cu, Cr, Hg, Ni, Pb, Sb, Se, Tl, and Zn). Of the remaining priority pollutant metals, beryllium was not investigated, while silver and arsenic did not form complexes with DBDC. In addition, one potential interference, manganese, can be determined. Cobalt is added as an internal standard. Separation of the complexes on a C18 column is achieved with a quaternary solvent system consisting of 64% methanol, 12% acetonitrile, 5% tetrahydrofuran, and 19% pH 5 acetate buffer. Detection limits range from a low of 0.1 ppb (ng/mL) for selenium to a high of 1.8 ppb for thallium.
美国环境保护局（EPA）目前指定原子吸收分光光度法作为优先污染物金属的分析方法。虽然该方法非常灵敏，但确实存在局限性。据报道，作为一种替代方法，使用二苄基二硫代氨基甲酸盐（DBDC）与样品中的优先污染物金属络合，随后通过液相色谱分离络合物并通过紫外可见分光光度法定量。在13种优先污染物金属中，有10种可以用该方法测定（镉、铜、铬、汞、镍、铅、锑、硒、铊和锌）。在其余的优先污染物金属中，未对铍进行研究，而银和砷未与DBDC形成络合物。此外，可以测定一种潜在干扰物锰。加入钴作为内标。在C18柱上使用由64%甲醇、12%乙腈、5%四氢呋喃和19%pH5乙酸盐缓冲液组成的四元溶剂系统实现络合物的分离。检测限范围从硒的低至0.1 ppb（纳克/毫升）到铊的高至1.8 ppb。
