Nikishin D A, Semenova M N, Shmukler Iu B
Ontogenez. 2012 May-Jun;43(3):212-6.
Neurotransmitters (including serotonin and acetylcholine) perform a number of prenervous functions in early sea urchin development. To detect the particular receptor components involved in these processes, we carried out a database search and nucleotide sequences homologous to serotonin receptor type 4, and the alpha6- and alpha10-subunits of nicotinic acetylcholine receptor were found among EST-clones from early Paracentrotus lividus embryos. Expression of these transcripts during early development was demonstrated using RT-PCR. These results are the first molecular biology evidence ofserotonin and acetylcholine receptor expression in sea urchin early embryogenesis.
神经递质（包括血清素和乙酰胆碱）在海胆早期发育过程中发挥着多种神经前功能。为了检测参与这些过程的特定受体成分，我们进行了数据库搜索，发现来自早期紫球海胆胚胎的EST克隆中存在与血清素4型受体以及烟碱型乙酰胆碱受体的α6和α10亚基同源的核苷酸序列。利用逆转录聚合酶链反应（RT-PCR）证明了这些转录本在早期发育过程中的表达。这些结果是海胆早期胚胎发生过程中血清素和乙酰胆碱受体表达的首个分子生物学证据。
