Bavykin A S, Mishin D V, Karpukhin A V, Deriabin P G
Vopr Virusol. 2012 Mar-Apr;57(2):32-4.
The use of the RNA interference technique yielded data on the antiviral activity of small interfering RNA (siRNA) oligonucleotides against hepatitis C virus (HCV) infection in the pig embryo kidney (SPEV) cell cultures. The RNA interference technique is based on the specific recognition of the mRNA target by using the specially designed siRNA (19-22 bp) oligonucleotides. In particular, it was shown that siRNA added to the monolayer of HCV-infected SPEV cells resulted in the protection of the infected cells against the cytopathogenic activity of the virus. The results were confirmed in the experiments that demonstrated the ability of RNA oligonucleotides to reduce the production of infectious (cytopathogenic) HCV by infected SPEV cells in early-stage infection.
RNA干扰技术的应用产生了关于小干扰RNA（siRNA）寡核苷酸对猪胚胎肾（SPEV）细胞培养物中丙型肝炎病毒（HCV）感染的抗病毒活性的数据。RNA干扰技术基于使用专门设计的siRNA（19 - 22个碱基对）寡核苷酸对mRNA靶标的特异性识别。特别地，研究表明，添加到HCV感染的SPEV细胞单层中的siRNA可保护受感染细胞免受病毒的细胞致病活性影响。在实验中证实了这些结果，这些实验证明了RNA寡核苷酸在早期感染中降低受感染的SPEV细胞产生感染性（细胞致病）HCV的能力。
