Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, Nakamachi, Nara, Japan.
Plant Signal Behav. 2012 Aug;7(8):944-6. doi: 10.4161/psb.20906. Epub 2012 Jul 27.
Reactive oxygen species (ROS) act as signaling molecules for regulating plant responses to abiotic and biotic stress and there exist source- and kind-specific pathways for ROS signaling. Recently, we created a novel system for producing H 2O 2 in Arabidopsis chloroplasts by chemical-dependent thylakoid membrane-bound ascorbate peroxidase (tAPX) silencing using an estrogen-inducible RNAi method. Microarray analysis revealed that the expression of a large set of genes was altered in response to tAPX silencing, some of which are known to be involved in pathogen response/resistance. Furthermore, we found that tAPX silencing enhances the levels of salicylic acid (SA) and the response to SA, a central regulator for biotic stress response. In this addendum, we describe the relationship between chloroplastic H 2O 2 and SA in stress response, and discuss the function of the kind- and source-specific ROS signaling in SA-mediated stress response.
活性氧（ROS）作为信号分子，调节植物对非生物和生物胁迫的响应，并且存在ROS 信号的源和种类特异性途径。最近，我们使用雌激素诱导的 RNAi 方法创建了一种通过化学依赖性类囊体膜结合抗坏血酸过氧化物酶（tAPX）沉默在拟南芥叶绿体中产生 H2O2 的新系统。微阵列分析显示，tAPX 沉默会导致大量基因的表达发生改变，其中一些基因已知参与了病原体的响应/抗性。此外，我们发现 tAPX 沉默会增强水杨酸（SA）的水平和对 SA 的响应，SA 是生物胁迫响应的中央调节剂。在本附录中，我们描述了叶绿体 H2O2 和 SA 在胁迫响应中的关系，并讨论了种类和源特异性 ROS 信号在 SA 介导的胁迫响应中的功能。
