一种基于靶标激活的自催化 DNA 酶扩增策略，用于测定碱基切除修复酶活性。
A target-activated autocatalytic DNAzyme amplification strategy for the assay of base excision repair enzyme activity.
机构信息
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
出版信息
Chem Commun (Camb). 2012 Sep 11;48(70):8820-2. doi: 10.1039/c2cc34531e. Epub 2012 Jul 27.
PMID:22836748
Abstract
Based on a target-activated autocatalytic DNAzyme amplification strategy, novel fluorescence sensing platforms were constructed for highly sensitive and selective assay of base excision repair enzyme activity. By using a rolling circle amplification (RCA)-coupled amplification cascade, an extremely low detection limit (0.002 U mL(-1)) was achieved.
摘要
基于靶标激活的自催化 DNA 酶扩增策略，构建了新型荧光传感平台，用于高灵敏度和选择性测定碱基切除修复酶活性。通过使用滚环扩增（RCA）偶联的扩增级联，实现了极低的检测限（0.002 U mL(-1))。
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