College of Engineering, The University of Georgia, Athens, GA 30602, USA.
Appl Biochem Biotechnol. 2012 Oct;168(3):519-30. doi: 10.1007/s12010-012-9793-6. Epub 2012 Jul 27.
In order to maximize microalgae biomass production and reduce its overall costs, it is important to optimize inoculum conditions based on its physical and physiological characteristics. Chlorella sorokiniana cultures inoculated with inoculum at three different physiological stages (lag, exponential, and stationary) diluted to the same optical density were cultivated for 12 days under three different CO(2) concentrations (0.038, 5, or 10 % CO(2) v/v) and growth pattern and biomass production was observed. Samples inoculated with lag phase inoculum supplied with 5 % CO(2) achieved the maximum biomass production, whereas samples supplied with 0.038 % CO(2) never reached exponential growth. The better growth of samples inoculated with lag phase inoculum was attributed to its increased number of cells compared to the other two inocula.
为了最大限度地提高微藻生物量的产量并降低其总成本，根据其物理和生理特性优化接种条件非常重要。将处于三种不同生理阶段（迟滞期、指数期和静止期）的接种物以三种不同的二氧化碳浓度（0.038%、5%或 10% v/v）稀释至相同的光密度，然后培养 12 天，并观察生长模式和生物量的产生。用 5%的二氧化碳供给处于迟滞期的接种物的样品达到了最大的生物量生产，而用 0.038%的二氧化碳供给的样品从未达到指数生长。与其他两种接种物相比，处于迟滞期的接种物的细胞数量更多，这也是其生长更好的原因。
