Department of Prosthodontics, School & Hospital of Stomatology, Wenzhou Medical College, Wenzhou 325027, China.
Carbohydr Polym. 2012 Oct 1;90(2):1024-31. doi: 10.1016/j.carbpol.2012.06.037. Epub 2012 Jun 21.
In this paper, the carboxymethyl chitosan/oxidized dextran hydrogel was developed and its potency application in the prevention of postoperative adhesion was investigated. The developed hydrogel showed porous and interconnected interior structure with pore size about 250 μm, which was sensitive to lysozymic solution (1.5 μg/ml) with almost complete degradation after 4 weeks of in vitro incubation. In vivo study suggested that the developed hydrogel showed the great capacity on the prevention of postoperative adhesions in rat model. According to the result of histopathological examination, it clearly showed that the mesothelial cell layer of abdominal wall and cecum were completely recovered after 7 days of surgery in 3% carboxymethyl chitosan/oxidized dextran hydrogel group, while obvious adhesion between abdominal wall and cecum was observed as treatment with saline solution or 3% carboxymethyl chitosan solution after 1 day of surgery. All these results suggested that the developed biodegradable hydrogel might have potential application in the prevention of postoperative adhesion.
本文开发了羧甲基壳聚糖/氧化葡聚糖水凝胶，并研究了其在预防术后粘连方面的潜在应用。所开发的水凝胶具有多孔且相互连通的内部结构，孔径约为 250μm，对溶菌酶溶液（1.5μg/ml）具有敏感性，在体外孵育 4 周后几乎完全降解。体内研究表明，该水凝胶在预防大鼠模型术后粘连方面具有很大的潜力。根据组织病理学检查结果，在 3%羧甲基壳聚糖/氧化葡聚糖水凝胶组中，术后 7 天腹壁和盲肠的间皮细胞层完全恢复，而在术后 1 天用生理盐水或 3%羧甲基壳聚糖溶液处理时，明显观察到腹壁和盲肠之间的粘连。所有这些结果表明，所开发的可生物降解水凝胶可能具有预防术后粘连的潜在应用。
