Yoon Tae Mi, Kim Sun-Ae, Lee Joon Kyoo, Park Young-Lan, Kim Gyu Yeol, Joo Young-Eun, Lee Jae Hyuk, Kim Kyung Keun, Lim Sang Chul
Department of Otolaryngology-Head and Neck Surgery, Research Institute of Medical Sciences, Chonnam National University Medical School & Hwasun Hospital, Gwangju, Republic of Korea.
Auris Nasus Larynx. 2013 Apr;40(2):222-6. doi: 10.1016/j.anl.2012.07.006. Epub 2012 Jul 25.
KAI1 COOH-terminal interacting tetraspanin (KITENIN) contributes to tumor invasion and metastasis in various cancers. The aim of this study was to investigate expression of KITENIN in patients with oral cavity squamous cell carcinoma (SCC) and to determine whether KITENIN affects tumor cell behavior in oral cavity SCC cell line.
Western blotting and immunohistochemistry was used to assess alteration of KITENIN expression in human oral cavity SCC and normal oral cavity mucosa. To evaluate the impact of KITENIN knockdown, the cell invasion assay and cell migration assay using small-interfering RNA were performed.
KITENIN protein expression was significantly increased in human oral cavity SCC tissues than in normal oral cavity mucosa by Western blotting. KITENIN immunoreactivity was strongly identified in human oral cavity SCC relative to adjacent normal tissue. Knockdown of KITENIN resulted in significantly reduced cell invasion in human oral cavity SCC cells (p=0.001). Cell migration showed a marked decrease in KITENIN knockdown oral cavity SCC cells compared to the negative control oral cavity SCC cells (p=0.01).
KITENIN is associated with tumor invasiveness and metastasis in human oral cavity SCC.
KAI1羧基末端相互作用四跨膜蛋白（KITENIN）在多种癌症中促进肿瘤侵袭和转移。本研究旨在调查KITENIN在口腔鳞状细胞癌（SCC）患者中的表达情况，并确定KITENIN是否影响口腔SCC细胞系中的肿瘤细胞行为。
采用蛋白质免疫印迹法和免疫组织化学法评估KITENIN在人口腔SCC和正常口腔黏膜中的表达变化。为评估KITENIN基因敲低的影响，使用小干扰RNA进行细胞侵袭试验和细胞迁移试验。
蛋白质免疫印迹法显示，人口腔SCC组织中KITENIN蛋白表达显著高于正常口腔黏膜。相对于相邻正常组织，在人口腔SCC中强烈鉴定出KITENIN免疫反应性。KITENIN基因敲低导致人口腔SCC细胞的细胞侵袭显著降低（p = 0.001）。与阴性对照口腔SCC细胞相比，KITENIN基因敲低的口腔SCC细胞的细胞迁移显著减少（p = 0.01）。
KITENIN与人口腔SCC中的肿瘤侵袭性和转移相关。
