Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, Via Ponte Don Melillo, 84084 Fisciano, Salerno, Italy.
J Pharm Biomed Anal. 2012 Nov;70:224-30. doi: 10.1016/j.jpba.2012.07.001. Epub 2012 Jul 13.
A validated analytical method for the quantitative determination of seven chemical markers occurring in a hydroalcoholic extract of Vitex agnus-castus fruits by liquid chromatography electrospray triple quadrupole tandem mass spectrometry (LC/ESI/(QqQ)MSMS) is reported. To carry out a comparative study, five commercial food supplements corresponding to hydroalcoholic extracts of V. agnus-castus fruits were analysed under the same chromatographic conditions of the crude extract. Principal component analysis (PCA), based only on the variation of the amount of the seven chemical markers, was applied in order to find similarities between the hydroalcoholic extract and the food supplements. A second PCA analysis was carried out considering the whole spectroscopic data deriving from liquid chromatography electrospray linear ion trap mass spectrometry (LC/ESI/(LIT)MS) analysis. High similarity between the two PCA was observed, showing the possibility to select one of these two approaches for future applications in the field of comparative analysis of food supplements and quality control procedures.
本文报道了一种采用液相色谱-电喷雾三重四极杆串联质谱（LC/ESI/(QqQ)MSMS）对牡荆果实水醇提取物中七种化学标志物进行定量测定的分析方法。为了进行比较研究，在相同的粗提物色谱条件下，对五种对应牡荆果实水醇提取物的商业食品补充剂进行了分析。主成分分析（PCA）仅基于七种化学标志物含量的变化，用于寻找水醇提取物和食品补充剂之间的相似性。考虑到来自液相色谱-电喷雾线性阱质谱（LC/ESI/(LIT)MS）分析的全光谱数据，进行了第二次 PCA 分析。观察到这两种 PCA 之间具有高度相似性，表明可以选择这两种方法中的一种用于食品补充剂比较分析和质量控制程序的未来应用。
