O'Rourke R W, Miller C W, Kato G J, Simon K J, Chen D L, Dang C V, Koeffler H P
Division of Hematology/Oncology, UCLA School of Medicine 90024-1678.
Oncogene. 1990 Dec;5(12):1829-32.
The human p53 gene codes for a 393 amino acid nuclear phosphoprotein. p53 is most commonly described as a tumor suppressor, or anti-oncogene, although its role in vivo remains unclear. We report that GAL4-p53 fusion protein can activate transcription of a CAT reporter gene downstream of a GAL4-DNA binding site. We tested both the amino terminal 160 amino acids and the carboxyl terminal 233 amino acids of the p53 protein and found that the transcriptional activating (TA) region was restricted to the amino terminal fragment. These results imply that p53 may be a transcriptional activating factor (TAF); furthermore, these data lend support to the hypothesis of p53 as a positive regulator of transcription which might mediate its tumor suppressor role by inducing expression of a set of genes with a negative effect on cellular growth.
人类p53基因编码一种含393个氨基酸的核磷蛋白。p53最常被描述为一种肿瘤抑制因子或抗癌基因，尽管其在体内的作用仍不清楚。我们报告称，GAL4-p53融合蛋白可激活GAL4-DNA结合位点下游的CAT报告基因的转录。我们测试了p53蛋白的氨基末端160个氨基酸和羧基末端233个氨基酸，发现转录激活（TA）区域局限于氨基末端片段。这些结果表明p53可能是一种转录激活因子（TAF）；此外，这些数据支持了p53作为转录正调节因子的假说，该因子可能通过诱导一组对细胞生长有负面影响的基因的表达来介导其肿瘤抑制作用。
