Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan.
Talanta. 2012 Aug 15;97:262-6. doi: 10.1016/j.talanta.2012.04.028. Epub 2012 Apr 26.
Oysters are one of foodstuffs containing a relatively high amount of cadmium. Here we report on establishment of an immunochromatographic assay (ICA) method of cadmium levels in oysters. Cadmium was extracted with 0.l mol L(-1) HCl from oysters and cleaned up from other metals by the use of an anion-exchange column. The behavior of five metals Mn, Fe, Cu, Zn, and Cd was monitored at each step of extraction and clean-up procedure for the ICA method in an inductively coupled plasma-mass spectrometry (ICP-MS) analysis. The results revealed that a simple extraction method with the HCl solution was efficient enough to extract almost all of cadmium from oysters. Clean-up with an anion-exchange column presented almost no loss of cadmium adsorbed on the column and an efficient removal of metals other than cadmium. When a spiked recovery test was performed in the ICA method, the recovery ranged from 98% to 112% with relative standard deviations between 5.9% and 9.2%. The measured values of cadmium in various oyster samples in the ICA method were favorably correlated with those in ICP-MS analysis (r(2)=0.97). Overall results indicate that the ICA method established in the present study is an adequate and reliable detection method for cadmium levels in oysters.
牡蛎是含镉量相对较高的食品之一。本研究建立了一种用于检测牡蛎中镉含量的免疫层析分析（ICA）方法。采用 0.1mol/L HCl 从牡蛎中提取镉，并用阴离子交换柱从其他金属中分离镉。在电感耦合等离子体质谱（ICP-MS）分析中，监测了五种金属 Mn、Fe、Cu、Zn 和 Cd 在提取和净化过程中的行为。结果表明，HCl 溶液的简单提取方法足以从牡蛎中提取几乎所有的镉。阴离子交换柱的净化几乎没有损失吸附在柱上的镉，并且可以有效地去除除镉以外的金属。在 ICA 方法中进行的加标回收试验中，回收率在 98%至 112%之间，相对标准偏差在 5.9%至 9.2%之间。ICA 方法测定的各种牡蛎样品中的镉含量与 ICP-MS 分析结果（r(2)=0.97）具有良好的相关性。总的来说，研究结果表明，本研究建立的 ICA 方法是一种用于检测牡蛎中镉含量的有效且可靠的检测方法。
