Faculty of Agronomy, Universidad de Ciego de Ávila, Ciego de Ávila, 69450, Cuba.
Plant Cell Rep. 2012 Nov;31(11):2065-73. doi: 10.1007/s00299-012-1317-x. Epub 2012 Jul 28.
In this work, we studied the effects of cryopreservation on various parameters of early stages of germination of Phaseolus vulgaris seeds (0, 7 and 14 days). Percentages of germination, fresh mass of different plant parts, levels of chlorophyll pigments (a, b, total), malondialdehyde, other aldehydes, phenolics (cell wall-linked, free, and total) and protein were determined. No phenotypic changes were observed visually in seedlings recovered from cryopreserved seeds. However, several significant effects of seed liquid nitrogen exposure were recorded at the biochemical level. There was a significant negative effect of cryopreservation on shoot protein content, which decreased from 3.11 mg g(-1) fresh weight for non-cryopreserved controls to 0.44 mg g(-1) fresh shoot weight for cryopreserved seeds. On the other hand, cryopreservation significantly increased levels of other aldehydes than malondialdehyde in shoots at day 7, from 56.47 μmol g(-1) for non-cryopreserved controls to 253.19 μmol g(-1) fresh shoot weight for cryopreserved samples. Liquid nitrogen exposure significantly reduced phenolics contents (free, cell-wall linked, total) in roots at day 7 after onset of germination. In general, roots were more affected by cryostorage compared with other plant parts, while leaves were the least affected. The effects of seed cryopreservation seem to decline progressively along with seedling growth.
在这项工作中，我们研究了冷冻保存对菜豆种子发芽早期（0、7 和 14 天）各个参数的影响。测定了发芽率、不同植物部位的鲜重、叶绿素色素（a、b、总）、丙二醛、其他醛类、酚类（细胞壁结合、游离和总）和蛋白质的水平。从冷冻保存的种子中恢复的幼苗在外观上没有观察到表型变化。然而，在生化水平上记录到了种子液氮气暴露的几个显著影响。冷冻保存对幼苗蛋白质含量有显著的负面影响，从非冷冻保存对照的 3.11mg g(-1)鲜重下降到冷冻保存种子的 0.44mg g(-1)鲜重。另一方面，在第 7 天，冷冻保存显著增加了茎中丙二醛以外的其他醛类的水平，从非冷冻保存对照的 56.47μmol g(-1)增加到冷冻保存样品的 253.19μmol g(-1)鲜重。液氮气暴露在萌发开始后的第 7 天显著降低了根中游离态、细胞壁结合态和总酚类物质的含量。总的来说，与其他植物部位相比，冷冻保存对根的影响更大，而对叶片的影响最小。种子冷冻保存的影响似乎随着幼苗生长而逐渐减弱。
