Ma T H, Xiong Q H, Yuan B, Jiang H, Gao Y, Xu J B, Liu S Y, Ding Y, Zhang G L, Zhao Y M, Zhang J B
College of Animal Science and Veterinary Medicine, Jilin University, Changchun, China.
Genet Mol Res. 2012 Jun 29;11(2):1721-30. doi: 10.4238/2012.June.29.4.
The luteinizing hormone receptor (LHR) plays a key role in testosterone production through its interaction with the gonadotropins, LH and chorionic gonadotropin. We examined the LHR splicing pattern in bovine Leydig cells; LH-induced expression of eight cloned splicing variants was detected by real-time PCR. Luteinizing hormone applied to cultured Leydig cells resulted in expression of full-length LHR and the A and B isoforms, as well as secretion of testosterone, which first increased, then declined, and then increased further, with increased LH levels. The secretion of testosterone progressively increased with increasing LH, but the expression levels of LHR (FL, A, and B) did not increase correspondingly. We conclude that the LHR splicing pattern is complex in bovine Leydig cells, and that expression of full-length LHR and isoforms A and B changes when induced with LH.
促黄体生成素受体（LHR）通过与促性腺激素、促黄体生成素（LH）和绒毛膜促性腺激素相互作用，在睾酮生成中起关键作用。我们检测了牛睾丸间质细胞中的LHR剪接模式；通过实时PCR检测到LH诱导的8种克隆剪接变体的表达。应用于培养的睾丸间质细胞的促黄体生成素导致全长LHR以及A和B亚型的表达，以及睾酮的分泌，随着LH水平的升高，睾酮分泌先增加，然后下降，随后进一步增加。睾酮的分泌随着LH的增加而逐渐增加，但LHR（全长、A和B）的表达水平并未相应增加。我们得出结论，牛睾丸间质细胞中的LHR剪接模式复杂，并且当用LH诱导时，全长LHR以及A和B亚型的表达会发生变化。
