Key Laboratory of Microbial Metabolism, Ministry of Education, Luc Montagnier Biomedical Research Institute, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Interdiscip Sci. 2012 Jun;4(2):83-9. doi: 10.1007/s12539-012-0125-x. Epub 2012 Jul 29.
The cytochrome P450 (CYP) enzymes play the central role in synthesis of endogenous substances and metabolism of xenobiotics. The substitution of single amino acid caused by non-synonymous single nucleotide polymorphism (nsSNP) will lead to the change in enzymatic activity of CYP isozymes, especially the drugmetabolizing ability. CYP-nsSNP is a specialized database focused on the effect of nsSNPs on enzymatic activity of CYPs. Its unique feature lies in providing the qualitative and quantitative description of the CYP variants in terms of enzymatic activity. In addition, the database also offers the general information about nsSNP and compounds that are involved in corresponding enzymatic reaction. The current CYP-nsSNP can be accessible at http://cypdatabase.sjtu.edu.cn/ and includes more than 300 genetic variants of 12 CYP isozymes together with about 100 compounds. In order to keep the accuracy of information within database, all experimental data were collected from the scientific literatures, and the users who conducted research to identify the novel CYP variants are encouraged to contribute their data. Therefore, CYP-nsSNP can be considered as a valuable source for experimental and computational studies of impact of genetic polymorphism on the function of CYPs.
细胞色素 P450（CYP）酶在合成内源性物质和代谢外源物质方面发挥着核心作用。由于非同义单核苷酸多态性（nsSNP）导致的单个氨基酸取代将导致 CYP 同工酶的酶活性发生变化，特别是药物代谢能力。CYP-nsSNP 是一个专注于 nsSNP 对 CYP 酶酶活性影响的专门数据库。其独特之处在于，它以酶活性的方式提供了对 CYP 变体的定性和定量描述。此外，该数据库还提供了关于 nsSNP 和参与相应酶反应的化合物的一般信息。目前，可在 http://cypdatabase.sjtu.edu.cn/ 访问 CYP-nsSNP，其中包含 12 种 CYP 同工酶的 300 多种遗传变体，以及约 100 种化合物。为了确保数据库内信息的准确性，所有实验数据均来自科学文献，鼓励进行鉴定新 CYP 变体的研究人员贡献他们的数据。因此，CYP-nsSNP 可以被视为研究遗传多态性对 CYP 功能影响的实验和计算研究的有价值资源。
