Gilbert Jack, Li Luen-Luen, Taghavi Safiyh, McCorkle Sean M, Tringe Susannah, van der Lelie Daniel
Department of Ecology & Evolution, University of Chicago, Chicago, IL, USA.
Methods Mol Biol. 2012;908:141-51. doi: 10.1007/978-1-61779-956-3_14.
To efficiently deconstruct recalcitrant plant biomass to fermentable sugars in industrial processes, biocatalysts of higher performance and lower cost are required. The genetic diversity found in the metagenomes of natural microbial biomass decay communities may harbor such enzymes. The aim of this chapter is to describe strategies, based on metagenomic approaches, for the discovery of glycoside hydrolases (GHases) from microbial biomass decay communities, especially those from unknown or never-been-cultivated microorganisms.
为了在工业过程中有效地将顽固的植物生物质解构为可发酵糖，需要高性能和低成本的生物催化剂。在天然微生物生物质降解群落的宏基因组中发现的遗传多样性可能包含此类酶。本章的目的是描述基于宏基因组学方法，从微生物生物质降解群落，特别是从未知或未培养微生物中发现糖苷水解酶（GHases）的策略。
