Wang Xiaoke, Ge Aiguo, Cheng Mengjie, Guo Fangfang, Zhao Min, Zhou Xiaoqi, Liu Liegang, Yang Nianhong
Department of Occupational and Environment Health, School of Public Health, Nantong University, Nantong 226019, China.
Exp Diabetes Res. 2012;2012:847246. doi: 10.1155/2012/847246. Epub 2012 Jul 11.
Inflammation has been implicated in the hypothalamic leptin and insulin resistance resulting defective food intake during high fat diet period. To investigate hypothalamic inflammation in dietary induced obesity (DIO) and obesity resistant (DIO-R) rats, we established rat models of DIO and DIO-R by feeding high fat diet for 10 weeks. Then we switched half of DIO and DIO-R rats to chow food and the other half to high fat diet for the following 8 weeks to explore hypothalamic inflammation response to the low fat diet intervention. Body weight, caloric intake, HOMA-IR, as well as the mRNA expression of hypothalamic TLR4, NF-κB, TNF-α, IL-1β, and IL-6 in DIO/HF rats were significantly increased compared to DIO-R/HF and CF rats, whereas IL-10 mRNA expression was lower in both DIO/HF and DIO-R/HF rats compared with CF rats. Switching to chow food from high fat diet reduced the body weight and improved insulin sensitivity but not affecting the expressions of studied inflammatory genes in DIO rats. Take together, upregulated hypothalamic inflammation may contribute to the overeating and development of obesity susceptibility induced by high fat diet. Switching to chow food had limited role in correcting hypothalamic inflammation in DIO rats during the intervention period.
炎症与下丘脑瘦素及胰岛素抵抗有关，在高脂饮食期间会导致食物摄入缺陷。为了研究饮食诱导肥胖（DIO）大鼠和肥胖抵抗（DIO-R）大鼠的下丘脑炎症，我们通过喂食高脂饮食10周建立了DIO和DIO-R大鼠模型。然后，我们将一半的DIO和DIO-R大鼠换成普通食物，另一半继续喂食高脂饮食，持续8周，以探究下丘脑对低脂饮食干预的炎症反应。与DIO-R/HF和CF大鼠相比，DIO/HF大鼠的体重、热量摄入、HOMA-IR以及下丘脑TLR4、NF-κB、TNF-α、IL-1β和IL-6的mRNA表达显著增加，而与CF大鼠相比，DIO/HF和DIO-R/HF大鼠的IL-10 mRNA表达均较低。从高脂饮食换成普通食物可降低DIO大鼠的体重并改善胰岛素敏感性，但不影响所研究的炎症基因的表达。综上所述，下丘脑炎症上调可能导致高脂饮食引起的暴饮暴食和肥胖易感性发展。在干预期间，换成普通食物对纠正DIO大鼠的下丘脑炎症作用有限。
