核因子 kappa B 参与大鼠高脂饮食相关的胰腺纤维化。
Involvement of nuclear factor kappa B in high-fat diet-related pancreatic fibrosis in rats.
机构信息
Department of Gastroenterology, Shandong Qianfoshan Hospital, Shandong University School of Medicine, Shandong, China.
出版信息
Gut Liver. 2012 Jul;6(3):381-7. doi: 10.5009/gnl.2012.6.3.381. Epub 2012 May 22.
BACKGROUND/AIMS: High-fat diets contribute to pancreatic fibrogenesis, but the pathogenesis remains unclear. This study investigated the role of nuclear factor kappa B (NF-κB) in high-fat diet-induced pancreatic fibrosis in rats.
METHODS
Male Wistar rats were fed a high-fat diet or standard normal chow for 20 weeks. Pancreatic fibrosis was determined by Sirius red staining. Immunohistochemical staining, reverse transcription-polymerase chain reaction and Western blotting were used to identify NF-κB-associated genes or protein expressions.
RESULTS
Inflammation, fat deposition, pancreatic stellate cell activation and fibrosis were observed in the pancreases of the high-fat diet group. NF-κB subunit p65 (NF-κB/p65) expression was localized to the nucleus, and intercellular adhesion molecule 1 (ICAM-1) was over-expressed. Pancreatic gene expression levels of NF-κB/p65, ICAM-1 and tumor necrosis factor α were all elevated significantly in rats fed a high-fat diet compared with control rats. Western blotting also revealed significantly increased levels of ICAM-1 and nuclear NF-κB/p65 in rats fed high-fat diets comparison with control rats.
CONCLUSIONS
NF-κB is involved in high-fat diet-related pancreatic fibrosis.
背景/目的：高脂肪饮食可导致胰腺纤维化，但发病机制尚不清楚。本研究旨在探讨核因子 κB（NF-κB）在高脂饮食诱导大鼠胰腺纤维化中的作用。
方法
雄性 Wistar 大鼠分别给予高脂肪饮食或标准正常饲料 20 周。采用天狼猩红染色法检测胰腺纤维化。免疫组织化学染色、逆转录-聚合酶链反应和 Western blot 检测 NF-κB 相关基因或蛋白的表达。
结果
高脂肪饮食组大鼠的胰腺可见炎症、脂肪沉积、胰腺星状细胞活化和纤维化。NF-κB 亚单位 p65（NF-κB/p65）表达定位于细胞核，细胞间黏附分子 1（ICAM-1）表达上调。与对照组相比，高脂肪饮食组大鼠胰腺 NF-κB/p65、ICAM-1 和肿瘤坏死因子 α 的基因表达水平均显著升高。Western blot 也显示，与对照组相比，高脂肪饮食组大鼠的 ICAM-1 和核 NF-κB/p65 水平显著升高。
结论
NF-κB 参与了高脂肪饮食相关的胰腺纤维化。
Zotero 插件