Qiang Guan, Jhaveri Ravi
Division of Infectious Diseases, Department of Pediatrics, Duke University Medical Center, Durham, NC 27710, USA.
ISRN Gastroenterol. 2012;2012:176728. doi: 10.5402/2012/176728. Epub 2012 Jul 11.
Background. Hepatitis C virus (HCV) genotype 3 is known to cause steatosis (fatty liver) that is more frequent and severe than other genotypes. We previously identified sequence elements within genotype 3 HCV Core domain 3 that were sufficient for lipid accumulation. Aims. We examined various genotype 3 Core domains for lipid droplet localization and compared the lipid droplet binding regions of domain 2 with a genotype 1 isolate. Methods. We generated HCV Core domain constructs fused with green fluorescent protein and performed immunofluorescence to visualize lipid droplets. Results. Constructs containing HCV Core domain 2 are appropriately localized to lipid droplets with varying degrees of efficiency. When compared to genotype 1, there are polymorphisms within domain 2 that do not appear to alter lipid droplet localization. Conclusions. In summary, the differences in a steatosis-associated HCV Core genotype 3 isolate do not appear to involve altered lipid droplet localization.
背景。已知丙型肝炎病毒（HCV）3型会导致脂肪变性（脂肪肝），相较于其他基因型，这种情况更常见且更严重。我们之前在3型HCV核心结构域3内鉴定出了足以导致脂质积累的序列元件。目的。我们研究了各种3型核心结构域的脂滴定位情况，并将结构域2的脂滴结合区域与1型分离株进行了比较。方法。我们构建了与绿色荧光蛋白融合的HCV核心结构域构建体，并进行免疫荧光以观察脂滴。结果。含有HCV核心结构域2的构建体以不同程度的效率正确定位于脂滴。与1型相比，结构域2内存在多态性，但似乎并未改变脂滴定位。结论。总之，与脂肪变性相关的HCV 3型核心分离株中的差异似乎并不涉及脂滴定位的改变。
