中国西南地区人类 MIF 水平、MIF-794CATT5-8 微卫星多态性与结核病易感性的关系研究。
Study of the relationship between human MIF level, MIF-794CATT5-8 microsatellite polymorphism, and susceptibility of tuberculosis in Southwest China.
机构信息
Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing Medical University, China.
出版信息
Braz J Infect Dis. 2012 Jul-Aug;16(4):383-6. doi: 10.1016/j.bjid.2012.06.018.
OBJECTIVE
To study the human migration inhibitory factor (MIF) level in tuberculosis (TB) patients, and the relationship between MIF-794CATT microsatellite polymorphism and susceptibility of TB in Southwest China.
METHODS
TB patients (n=151) and healthy unrelated controls (n=149) were recruited for this study. Genomic DNA was extracted, and then amplified by polymerase chain reaction (PCR). MIF-794CATT(5-8) microsatellite polymorphism was genotyped by DNA sequencing. MIF level was detected by ELISA.
RESULTS
In the TB group, the repeat number of 7/7 and 7/8 (17.89%) was significantly higher than that of the control group (8.05%), and the serum MIF level was also much higher than that of the healthy controls (705.21 ± 67.98 vs. 355.31 ± 57.29 pg/mL, p<0.01).
CONCLUSION
The appearance of MIF-794CATT 7/7 and 7/8 is associated with susceptibility to TB, and may play an important role in the occurrence and development of TB in Southwest China.
目的
研究人迁移抑制因子（MIF）在结核病（TB）患者中的水平，以及 MIF-794CATT 微卫星多态性与中国西南地区 TB 易感性的关系。
方法
本研究招募了 151 例 TB 患者和 149 例健康无关对照者。提取基因组 DNA，然后通过聚合酶链反应（PCR）扩增。通过 DNA 测序对 MIF-794CATT（5-8）微卫星多态性进行基因分型。通过 ELISA 检测 MIF 水平。
结果
在 TB 组中，7/7 和 7/8 重复数（17.89%）明显高于对照组（8.05%），血清 MIF 水平也明显高于健康对照组（705.21±67.98 vs. 355.31±57.29 pg/mL，p<0.01）。
结论
MIF-794CATT7/7 和 7/8 的出现与 TB 的易感性有关，可能在中国西南地区 TB 的发生和发展中起重要作用。
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