Niu Hong-xin, Du Tong, Xu Zhong-fa, Zhang Xi-kun, Wang Ruo-gu
Department of General Surgery, Affiliated Hospital of Shandong Academy of Medical Science, PR, China.
Acta Cir Bras. 2012 Aug;27(8):522-8. doi: 10.1590/s0102-86502012000800002.
To investigate the feasibility of interventional lipiodol embolism and multigene therapy in combination with focal chemotherapy in the treatment of VX2 liver cancer in rabbits.
Forty five rabbits with cancer larger than 2cm in diameter were randomly divided into five groups (n=9 per group). In Group 1, animals were treated with 0.9% sodium chloride. In Group 2, animals received lipiodol embolism. In Group 3, animals received lipiodol embolism and p53 gene therapy. In Group 4, animals received lipiodol embolism and TK/CD gene therapy. In Group 5, animals received lipiodol embolism and p53 and TK/CD gene therapy. Ultrasonography and CT were performed before and at ten days after interventional therapy.
The VX2 model of liver cancer was successfully established in rabbits and interventional therapy smoothly performed. At ten days after interventional therapy, significant difference in the tumor volume was noted among five groups (p<0.05) and different treatments could inhibit the cancer growth. The inhibition of cancer growth was the most evident in the Group 5. Factorial analysis revealed gene therapy with p53 or TK/CD and lipiodol embolism independently exert significantly inhibitory effect on cancer growth. In addition, the suppression on tumor growth rate was the most obvious in the Group 5.
Combination of gene therapy with lipiodol embolism can effectively inhibit the cancer growth and prolong the survival time. These findings demonstrate the effectiveness of multigene therapy in combination with lipiodol embolism in the treatment of liver cancer.
探讨碘油介入栓塞联合多基因治疗及局部化疗在兔VX2肝癌治疗中的可行性。
将45只直径大于2cm的荷瘤兔随机分为5组（每组n = 9）。第1组动物接受0.9%氯化钠治疗。第2组动物接受碘油栓塞。第3组动物接受碘油栓塞及p53基因治疗。第4组动物接受碘油栓塞及TK/CD基因治疗。第5组动物接受碘油栓塞及p53和TK/CD基因治疗。在介入治疗前及治疗后10天进行超声和CT检查。
成功建立兔VX2肝癌模型并顺利实施介入治疗。介入治疗后10天，5组间肿瘤体积差异有统计学意义（p<0.05），不同治疗方法均能抑制肿瘤生长。第5组对肿瘤生长的抑制作用最明显。析因分析显示，p53或TK/CD基因治疗与碘油栓塞均能独立对肿瘤生长产生显著抑制作用。此外，第5组对肿瘤生长速率的抑制作用最明显。
基因治疗联合碘油栓塞可有效抑制肿瘤生长并延长生存时间。这些结果表明多基因治疗联合碘油栓塞治疗肝癌有效。
