Luo Jun, Wang Xin-bao, Yu Qi-ming, Zhao Ting, Fang Ren-gui, Wang Jian-jun, Zheng Zhi-guo, Yu Jiang-liu, Fang Xian-hua, Wang Shi, Ling Zhi-qiang
Zhejiang Cancer Research Institute, Hangzhou, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2012 Jul;15(7):710-4.
To explore the association between the progression of gastric cancer and the aberrant methylation of CDH1 gene in preoperative abdominal lavage fluid.
Real-time methylation-specific polymerase chain reaction(qMSP) was used to investigate the methylation status of the CDH1 gene promoter 5'-CpG islands from preoperative abdominal lavage fluid in 92 patients with gastric cancer. The associations between methylation of CDH1 genes and clinicopathologic features and prognosis were investigated.
Among the 92 patients with gastric cancer, aberrant methylation of CDH1 gene was detected in 45(48.9%) patients, including total aberrant methylation in 12(13.0%) cases and partly aberrant methylation in 33(35.9%) cases. Significant associations were found between CDH1 methylation status and tumor size, growth pattern, differentiation, lymphovascular invasion, infiltration depth, lymph node metastasis, distant metastasis, and clinical staging(all P<0.05). However, there were no significant associations between CDH1 methylation status with gender, age, tumor location, or Helicobacter pylori infection(all P>0.05). The median progression-free survival was 20 months for CDH1 methylation group and 38 months for non-methylated group, and the difference was statistically significant(P<0.01). Cox model analysis revealed that CDH1 methylation status in preoperative peritoneal lavage fluid was an independent factor associated with postoperative survival in patients with gastric cancer(P=0.000, RR=332.88, 95%CI:21.71-5105.07).
The aberrant methylation of 5'-CpG of CDH1 gene promoter is common in gastric cancer. The examination of CDH1 methylation status of abdominal lavage should be considered in the progression of gastric cancer.
探讨术前腹腔灌洗液中胃癌进展与CDH1基因异常甲基化之间的关系。
采用实时甲基化特异性聚合酶链反应（qMSP）检测92例胃癌患者术前腹腔灌洗液中CDH1基因启动子5'-CpG岛的甲基化状态。研究CDH1基因甲基化与临床病理特征及预后的关系。
92例胃癌患者中，45例（48.9%）检测到CDH1基因异常甲基化，其中完全异常甲基化12例（13.0%），部分异常甲基化33例（35.9%）。CDH1甲基化状态与肿瘤大小、生长方式、分化程度、淋巴管浸润、浸润深度、淋巴结转移、远处转移及临床分期均有显著相关性（均P<0.05）。然而，CDH1甲基化状态与性别、年龄、肿瘤部位或幽门螺杆菌感染均无显著相关性（均P>0.05）。CDH1甲基化组的无进展生存期中位数为20个月，未甲基化组为38个月，差异有统计学意义（P<0.01）。Cox模型分析显示，术前腹腔灌洗液中CDH1甲基化状态是胃癌患者术后生存的独立相关因素（P=0.000，RR=332.88，95%CI：21.71-5105.07）。
CDH1基因启动子5'-CpG异常甲基化在胃癌中常见。在胃癌进展过程中应考虑检测腹腔灌洗液中CDH1甲基化状态。
