4-氧代戊烯二酸水合酶的非预期的混合活性：硝代苯乙烯的顺反异构化。
An unexpected promiscuous activity of 4-oxalocrotonate tautomerase: the cis-trans isomerisation of nitrostyrene.
机构信息
Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Groningen, The Netherlands.
出版信息
Chembiochem. 2012 Sep 3;13(13):1869-73. doi: 10.1002/cbic.201200371. Epub 2012 Jul 31.
PMID:22851288
Abstract
Serendipitous switch: While exploring cis-nitrostyrene as a potential electrophile in Michael-type addition reactions catalysed by the enzyme 4-oxalocrotonate tautomerase (4-OT), it was unexpectedly found that 4-OT catalyses the isomerisation of cis-nitrostyrene to trans-nitrostyrene (k(cat) /K(m) = 1.9×10(3) M(-1) s(-1) ).
摘要
意外转换
在探索顺式硝基苯乙烯作为酶 4-氧代戊烯酸转脒酶（4-OT）催化的迈克尔加成反应中的潜在亲电试剂时，出人意料地发现 4-OT 催化顺式硝基苯乙烯异构化为反式硝基苯乙烯（k(cat)/K(m) = 1.9×10(3) M(-1) s(-1)）。
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