Carter T P, Baek H K, Bonninghausen L, Morris R J, van Wart H E
Institute of Molecular Biophysics, Florida State University, Tallahassee 32306-3006.
Anal Biochem. 1990 Oct;190(1):134-40. doi: 10.1016/0003-2697(90)90146-z.
A 512-element diode-array spectroscopic detection system capable of acquiring multiple spectra at a rate of 5 ms per spectrum with an effective scan rate of 102.9 kHz has been constructed. Spectra with fewer diode elements can also be acquired at scan rates up to 128 kHz. The detector utilizes a Hamamatsu silicon photodiode-array sensor that is interfaced to Hamamatsu driver/amplifier and clock generator boards and a DRA laboratories 12-bit 160-kHz analog-to-digital converter. These are standard, commercially available devices which cost approximately $3500. The system is interfaced to and controlled by an IBM XT microcomputer. Detailed descriptions of the home-built detector housing and control/interface circuitry are presented and its application to the study of the reaction of horseradish peroxidase with hydrogen peroxide is demonstrated.
已经构建了一个512元件二极管阵列光谱检测系统，该系统能够以每光谱5毫秒的速率采集多个光谱，有效扫描速率为102.9千赫兹。二极管元件较少的光谱也可以在高达128千赫兹的扫描速率下采集。该探测器采用滨松硅光电二极管阵列传感器，该传感器与滨松驱动器/放大器和时钟发生器板以及DRA实验室的12位160千赫兹模数转换器相连。这些都是标准的商用设备，成本约为3500美元。该系统与IBM XT微型计算机相连并由其控制。文中给出了自制探测器外壳以及控制/接口电路的详细描述，并展示了其在辣根过氧化物酶与过氧化氢反应研究中的应用。
