采用高效液相色谱法测定天冬氨酰葡糖胺酶
Assay of aspartylglycosylaminase by high-performance liquid chromatography.
作者信息
Kaartinen V, Mononen I
机构信息
Department of Clinical Chemistry, Kuopio University Central Hospital, Finland.
出版信息
Anal Biochem. 1990 Oct;190(1):98-101. doi: 10.1016/0003-2697(90)90140-5.
PMID:2285152
Abstract
An aspartylglycosylaminase assay based on high-performance liquid chromatographic analysis of the substrate aspartylglucosamine and product aspartate is described. Aspartylglucosamine and aspartate are derivatized with phenylisothiocyanate and resolved by reverse-phase chromatography. The detection limit for the compounds is 2 pmol. The method can be used for analysis of aspartylglycosylaminase activity in crude cell extracts and tissue samples.
摘要
描述了一种基于对底物天冬氨酰葡糖胺和产物天冬氨酸进行高效液相色谱分析的天冬氨酰葡糖胺酶测定法。天冬氨酰葡糖胺和天冬氨酸用异硫氰酸苯酯衍生化，并用反相色谱法分离。这些化合物的检测限为2皮摩尔。该方法可用于分析粗细胞提取物和组织样品中的天冬氨酰葡糖胺酶活性。
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