Laboratory of Pharmacology, Department of Translational Pathophysiological Research, Faculty of Medicine and Health Sciences, University of Antwerp, Wilrijk, 2610 Antwerp, Belgium.
Mediators Inflamm. 2012;2012:690643. doi: 10.1155/2012/690643. Epub 2012 Jul 18.
Earlier investigations have revealed a surprising complexity and variety in the range of interaction between cells of the innate and adaptive immune system. Our understanding of the specialized roles of dendritic cell (DC) subsets in innate and adaptive immune responses has been significantly advanced over the years. Because of their immunoregulatory capacities and because very small numbers of activated DC are highly efficient at generating immune responses against antigens, DCs have been vigorously used in clinical trials in order to elicit or amplify immune responses against cancer and chronic infectious diseases. A better insight in DC immunobiology and function has stimulated many new ideas regarding the potential ways forward to improve DC therapy in a more fundamental way. Here, we discuss the continuous search for optimal in vitro conditions in order to generate clinical-grade DC with a potent immunogenic potential. For this, we explore the molecular and cellular mechanisms underlying adequate immune responses and focus on most favourable DC culture regimens and activation stimuli in humans. We envisage that by combining each of the features outlined in the current paper into a unified strategy, DC-based vaccines may advance to a higher level of effectiveness.
早期的研究揭示了先天免疫和适应性免疫系统细胞之间相互作用的惊人复杂性和多样性。近年来，我们对树突状细胞（DC）亚群在先天和适应性免疫反应中的特殊作用的理解有了显著的提高。由于其免疫调节能力，以及少量激活的 DC 就能高效地产生针对抗原的免疫反应，因此 DC 已被广泛用于临床试验，以引发或增强针对癌症和慢性传染病的免疫反应。对 DC 免疫生物学和功能的更深入了解，激发了许多关于以更根本的方式改善 DC 治疗的潜在方法的新想法。在这里，我们讨论了不断寻找最佳的体外条件，以生成具有强大免疫原性的临床级 DC。为此，我们探索了产生充分免疫反应的分子和细胞机制，并关注人类中最有利的 DC 培养方案和激活刺激物。我们设想，通过将当前论文中概述的每一个特征结合到一个统一的策略中，基于 DC 的疫苗可能会提高有效性。
