SNPforID分析作为亲缘关系和微量分析的补充方法。
The SNPforID Assay as a Supplementary Method in Kinship and Trace Analysis.
作者信息
Schwark Thorsten, Meyer Patrick, Harder Melanie, Modrow Jan-Hendrick, von Wurmb-Schwark Nicole
机构信息
Institute of Legal Medicine, University Hospital Schleswig-Holstein, Kiel, Germany.
出版信息
Transfus Med Hemother. 2012 Jun;39(3):187-193. doi: 10.1159/000338855. Epub 2012 May 11.
Abstract
OBJECTIVE
Short tandem repeat (STR) analysis using commercial multiplex PCR kits is the method of choice for kinship testing and trace analysis. However, under certain circumstances (deficiency testing, mutations, minute DNA amounts), STRs alone may not suffice. METHODS: We present a 50-plex single nucleotide polymorphism (SNP) assay based on the SNPs chosen by the SNPforID consortium as an additional method for paternity and for trace analysis. The new assay was applied to selected routine paternity and trace cases from our laboratory. RESULTS AND CONCLUSIONS: Our investigation shows that the new SNP multiplex assay is a valuable method to supplement STR analysis, and is a powerful means to solve complicated genetic analyses.
摘要
目的
使用商业多重聚合酶链反应（PCR）试剂盒进行短串联重复序列（STR）分析是亲缘关系检测和微量分析的首选方法。然而，在某些情况下（如缺乏检测、突变、DNA量极少），仅靠STR可能并不足够。方法：我们基于SNPforID联盟选择的单核苷酸多态性（SNP），提出了一种50重SNP检测方法，作为亲子鉴定和微量分析的附加方法。该新检测方法应用于我们实验室选定的常规亲子鉴定和微量案例。结果与结论：我们的研究表明，新的SNP多重检测方法是补充STR分析的有价值方法，也是解决复杂基因分析的有力手段。
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