Dept. of Entomology, Punjab Agricultural Univ., Ludhiana-141004, Punjab, India.
J Food Sci. 2012 Dec;77(12):T208-15. doi: 10.1111/j.1750-3841.2012.02801.x. Epub 2012 Jul 31.
An easy, simple and efficient analytical method was standardized and validated for the estimation of residues of chlorantraniliprole in different vegetables comprising brinjal, cabbage, capsicum, cauliflower, okra, and tomato. QuEChERS method was used for the extraction and cleanup of chlorantraniliprole residues on these vegetables. Final clear extracts of ethyl acetate were concentrated under vacuum and reconstituted into high performance liquid chromatograph (HPLC) grade acetonitrile, and residues were estimated using HPLC equipped with PDA detector system, C(18) column and confirmed by liquid chromatograph mass spectrometer (LC-MS/MS), and high performance thin layer chromatograph (HPTLC). HPLC grade acetonitrile:water (80:20, v/v) was used as mobile phase @ 0.4 mL/min. Chlorantraniliprole presented distinct peak at retention time of 9.82 min. Consistent recoveries ranging from 85% to 96% for chlorantraniliprole were observed when samples were spiked at 0.10, 0.25, 0.50, and 1.00 mg/kg levels. The limit of quantification of this method was worked out to be 0.10 mg/kg.
建立了一种简单、快速、有效的分析方法，用于测定茄子、白菜、辣椒、菜花、秋葵和番茄等不同蔬菜中氯虫苯甲酰胺的残留量。采用 QuEChERS 方法对蔬菜中的氯虫苯甲酰胺残留进行提取和净化。乙腈提取液在减压下浓缩，用高效液相色谱（HPLC）级乙腈重新溶解，采用配备 PDA 检测器系统、C（18）柱的高效液相色谱法（HPLC）进行残留量测定，并通过液相色谱-质谱联用仪（LC-MS/MS）和高效薄层色谱（HPTLC）进行确证。以 HPLC 级乙腈：水（80:20，v/v）为流动相，流速为 0.4 mL/min。氯虫苯甲酰胺在保留时间为 9.82 min 处有明显的峰。当样品在 0.10、0.25、0.50 和 1.00 mg/kg 水平下进行加标时，氯虫苯甲酰胺的回收率在 85%至 96%之间。该方法的定量限为 0.10 mg/kg。
