使用模块化适配体策略的 c-di-GMP 的纳摩尔荧光检测。
Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy.
机构信息
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
出版信息
Chem Commun (Camb). 2012 Sep 18;48(72):9059-61. doi: 10.1039/c2cc34379g. Epub 2012 Aug 2.
PMID:22854718
Abstract
C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spinach as the fluorescent reporting module.
摘要
c-di-GMP 调节几种细菌中生物膜形成和毒力因子产生所涉及的重要过程。在此，我们报告了一种简单的荧光策略，该策略使用 Vc2 类 I 核糖体开关结构域作为传感区域，菠菜作为荧光报告模块，可检测 c-di-GMP（低至 320 nM）。
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