两亲性聚合物对百日咳博德特氏菌生长及百日咳博德特氏菌血凝素产生的影响。

The effect of amphiphilic polymers on growth of Bordetella pertussis and production of B. pertussis haemagglutinins.

作者信息

Nikolajewski H E, Liepert H, Nikolajewski G, Pfüller U, Swidsinski S, Wettig G

机构信息

Staatliches Institut für Immunopräparate und Nährmedien, Berlin, D.D.R.

出版信息

Biologicals. 1990 Oct;18(4):305-8. doi: 10.1016/1045-1056(90)90034-w.

DOI:10.1016/1045-1056(90)90034-w

PMID:2285502

Abstract

The effect, upon addition to a culture medium of amphiphilic polymers such as partly hydrolysed polyvinyl acetate (PVAc) or partly methylated cellulose (MeCel) on growth and production of haemagglutinins--the filamentous haemagglutinin (FHA) and the pertussis toxin (PT)--has been investigated. As a result, an increase of HA-titer and PT could be achieved. Besides these effects the addition of the amphiphilic polymers stimulated growth of B. pertussis. The above results are comparable to, or even better than, those obtained by addition of Heptakis (2,6-0-dimethyl) beta-cyclodextrin (Me-beta-CD) or polyvinyul alcohols (PVA) which have already been described as compounds to enhance growth and yield of haemagglutinins.

摘要

研究了在培养基中添加两亲聚合物（如部分水解的聚醋酸乙烯酯（PVAc）或部分甲基化的纤维素（MeCel））对血凝素（丝状血凝素（FHA）和百日咳毒素（PT））生长和产生的影响。结果发现，可以实现血凝素效价和PT的增加。除了这些作用外，两亲聚合物的添加还刺激了百日咳博德特氏菌的生长。上述结果与添加七（2,6-O-二甲基）β-环糊精（Me-β-CD）或聚乙烯醇（PVA）所获得的结果相当，甚至更好，而Me-β-CD和PVA已被描述为可提高血凝素生长和产量的化合物。