Suzuki Y, Imaizumi A, Ginnaga A, Sato H, Sato Y
Dev Biol Stand. 1985;61:89-92.
Heptakis (2,6-0 dimethyl)beta-cyclodextrin (MeCD) which permits the growth of single colonies of Bordetella pertussis Tohama phase I on Stainer-Scholte medium solidified with agar also enhanced the production of pertussis toxin (PT). More than one hundred times the amount of PT was produced in Stainer-Scholte medium with MeCD in shake culture than was produced in MeCD-free medium. A maximum of 50 mg PT protein was produced per liter of culture broth as estimated by in vitro and in vivo assays. The production of filamentous hemagglutinin (FHA) was several hundred times greater when B. pertussis was grown in shake cultures with MeCD than when growth was in MeCD-free shake cultures. The FHA content of the production medium was confirmed by enzyme-linked immunosorbent assays and electron microscopy. Evaluation of an acellular vaccine containing PT and FHA detoxified with formaldehyde showed that it was protective in the intracerebral challenge mouse potency assay.
七（2,6-二-O-甲基）-β-环糊精（MeCD）能使百日咳博德特氏菌Tohama I相在琼脂固化的斯坦纳-斯科尔特培养基上形成单菌落，同时还能提高百日咳毒素（PT）的产量。在摇瓶培养中，添加MeCD的斯坦纳-斯科尔特培养基产生的PT量比无MeCD培养基高出一百多倍。通过体外和体内试验估计，每升培养液最多可产生50毫克PT蛋白。当百日咳博德特氏菌在添加MeCD的摇瓶培养中生长时，丝状血凝素（FHA）的产量比在无MeCD的摇瓶培养中高出数百倍。通过酶联免疫吸附测定和电子显微镜确认了生产培养基中的FHA含量。对含有经甲醛解毒的PT和FHA的无细胞疫苗进行评估，结果表明其在脑内攻击小鼠效力试验中具有保护作用。
