副百日咳博德特氏菌不耐热毒素细胞培养测定法的开发。

Development of a cell culture assay for Bordetella parapertussis heat-labile toxin.

作者信息

Endoh M, Nagai M, Burns D L, Manclark C R, Nakase Y

机构信息

Department of Microbiology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.

出版信息

Biologicals. 1990 Oct;18(4):309-13. doi: 10.1016/1045-1056(90)90035-x.

DOI:10.1016/1045-1056(90)90035-x

PMID:2285503

Abstract

A cell culture assay for heat-labile toxin isolated from Bordetella parapertussis has been developed. In this assay, the ability of heat-labile toxin to induce contraction of vascular smooth muscle cells is measured. The method allows for detection of as little as 0.6 ng/ml of the toxin. The results obtained from this in vitro assay correlated well with those obtained with in vivo assays indicating that the cell culture assay may be a useful alternative to animal assays.

摘要

已开发出一种用于检测从副百日咳博德特氏菌中分离出的热不稳定毒素的细胞培养测定法。在该测定法中，可测量热不稳定毒素诱导血管平滑肌细胞收缩的能力。该方法能够检测低至0.6 ng/ml的毒素。从这种体外测定法获得的结果与体内测定法获得的结果相关性良好，表明细胞培养测定法可能是动物测定法的一种有用替代方法。