Yu Yu, Suo Lun, Wu Qiang
Shanghai Jiao Tong University, Shanghai, China.
Dongwuxue Yanjiu. 2012 Aug;33(4):362-6. doi: 10.3724/SP.J.1141.2012.04362.
This work used Immunohistochemistry to examine the expression of myelin basic protein and accumulation of oligodendrocytes in Pchdα knockout and control littermate mice. Data showed that in Pchdα knockout mice, Myelin proteins decrease in the central nervous system and mature oligodendrocytes in the cerebellum also decrease. Furthermore, deletion of the Pcdhα cluster does not cause any change to the axons and astrocytes in quantification of relative marker proteins. These findings suggest that the Pcdhα cluster may be required for myelination and oligodendrite development of the brain in mice, and that Pcdhα cluster may play a key role in the development of the central nervous system.
这项研究使用免疫组织化学方法检测了Pchdα基因敲除小鼠和对照同窝小鼠中髓鞘碱性蛋白的表达以及少突胶质细胞的聚集情况。数据显示，在Pchdα基因敲除小鼠中，中枢神经系统中的髓鞘蛋白减少，小脑成熟少突胶质细胞也减少。此外，在相对标记蛋白定量分析中，Pcdhα基因簇的缺失对轴突和星形胶质细胞没有任何影响。这些发现表明，Pcdhα基因簇可能是小鼠大脑髓鞘形成和少突胶质细胞发育所必需的，并且Pcdhα基因簇可能在中枢神经系统发育中起关键作用。
