Yang Ming-Hua, Su Jin-Zhu, Liu Jun, Li Ya-Hui
College of Animal Science & Technology, Yunnan Agricultural University, Kunming, China.
Dongwuxue Yanjiu. 2012 Aug;33(4):381-8. doi: 10.3724/SP.J.1141.2012.04381.
We sought to experimentally verify if testis specific serine/threonine kinases (Tssks) play a role in spermatogenesis and/or the regulation of sperm function. Purified Tssk proteins were obtained based on cloning and expression of mouse Tssk1 and Tssk2. Tssk1 and Tssk2 were detected in mature mouse and human sperm by western blotting. Immunofluorescence indicated that Tssk1 is distributed in the acrosome and the entire flagellum of mouse sperm while Tssk2 was mainly distributed in post-acrosomal region. There was no alteration in the distribution pattern of Tssk1 and Tssk2 in non-capacitated and capacitated sperm. Tssk2 distribution remained unchanged after induced acrosome reaction but no signals were detected in the acrosome for Tssk1, which was present before the acrosome reaction, though signals in flagellum were undisturbed. In human sperm, Tssk1 was found in neck and flagellum while Tssk2 was found in the equatorial region. Our results suggest Tssk1 and/or Tssk2 do play an important role(s) in the regulation of sperm function.
我们试图通过实验验证睾丸特异性丝氨酸/苏氨酸激酶（Tssks）是否在精子发生和/或精子功能调节中发挥作用。基于小鼠Tssk1和Tssk2的克隆和表达获得了纯化的Tssk蛋白。通过蛋白质免疫印迹法在成熟的小鼠和人类精子中检测到了Tssk1和Tssk2。免疫荧光表明，Tssk1分布于小鼠精子的顶体和整个鞭毛，而Tssk2主要分布于顶体后区域。在未获能和获能精子中，Tssk1和Tssk2的分布模式没有改变。诱导顶体反应后，Tssk2的分布保持不变，但在顶体中未检测到Tssk1的信号，顶体反应前Tssk1存在于顶体中，不过鞭毛中的信号未受干扰。在人类精子中，Tssk1存在于颈部和鞭毛，而Tssk2存在于赤道区域。我们的结果表明，Tssk1和/或Tssk2在精子功能调节中确实发挥着重要作用。
