Coombes R C, Barrett-Lee P, Luqmani Y
Department of Medical Oncology, St. George's Hospital Medical School, London, England.
J Steroid Biochem Mol Biol. 1990 Dec 20;37(6):833-6. doi: 10.1016/0960-0760(90)90428-n.
We have studied mRNA levels for a variety of growth factors in biopsy specimens from malignant, benign and normal breast tissue. We found TGFb mRNA in all breast cancers and neoplastic breast tissues but the level of the TGFb mRNA were found to be higher in breast cancer (P = 0.01). TGFa mRNA was detected in a similar proportion of cancers as in neoplastic breast tissues but the TGFa receptor EGFR mRNA was detected in only 55% of breast cancers but in all non-neoplastic breast tissue tested. The presence of EGFR mRNA was inverted related to oestrogen receptor status and coexpression of TGFa and EGFR was observed in 28% of carcinomas, and significantly more commonly in ER negative tumours (P = 0.01). PDGF a and b chain transcripts coexisted in all normal and malignant breast tissue. Insulin-like growth factor II mRNA was present in all 15 samples of non-malignant breast tissue but in only 11 of 21 (52%) of carcinomas.
我们研究了来自恶性、良性和正常乳腺组织活检标本中多种生长因子的mRNA水平。我们在所有乳腺癌和乳腺肿瘤组织中均发现了转化生长因子β（TGFβ）mRNA，但发现乳腺癌中TGFβ mRNA的水平更高（P = 0.01）。在与乳腺肿瘤组织相似比例的癌症中检测到了转化生长因子α（TGFα）mRNA，但仅在55%的乳腺癌中检测到了TGFα受体表皮生长因子受体（EGFR）mRNA，而在所有检测的非肿瘤性乳腺组织中均检测到。EGFR mRNA的存在与雌激素受体状态呈负相关，在28%的癌中观察到TGFα和EGFR的共表达，且在雌激素受体阴性肿瘤中更常见（P = 0.01）。血小板衍生生长因子（PDGF）α和β链转录本在所有正常和恶性乳腺组织中共存。胰岛素样生长因子II mRNA存在于所有15份非恶性乳腺组织样本中，但仅在21份癌中的11份（52%）中存在。
