Division of Pulmonary and Critical Care, Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226.
Division of Pulmonary and Critical Care, Department of Pathology, Medical College of Wisconsin, Milwaukee, WI 53226.
Prostaglandins Leukot Essent Fatty Acids. 2012 Aug-Sep;87(2-3):79-89. doi: 10.1016/j.plefa.2012.07.002. Epub 2012 Aug 2.
The chick chorioallantoic membrane (CAM) subserves gas exchange in the developing embryo and shell-less culture affords a unique opportunity for direct observations over time of individual blood vessels to pharmacologic interventions. We tested a number of lipids including prostaglandins PGE(1&2) for vascular effects and signaling in the CAM. Application of PGE(1&2) induced a decrease in the diameter of large blood vessels and a concentration-dependent, localized, reversible loss of blood flow through small vessels. The loss of flow was also mimicked by misoprostol, an agonist for 3 of 4 known PGE receptors, EP(2-4), and by U46619, a thromboxane mimetic. Selective receptor antagonists for EP(3) and thromboxane each partially blocked the response. This is a first report of the effects of prostaglandins on vasoreactivity in the CAM. Our model allows the unique ability to examine simultaneous responses of large and small vessels in real time and in vivo.
鸡胚绒毛尿囊膜（CAM）为胚胎发育提供气体交换，而无壳培养为观察单个血管对药物干预的长期直接观察提供了独特的机会。我们测试了多种脂质，包括前列腺素 PGE（1 和 2），以观察其对 CAM 中的血管作用和信号传导的影响。PGE（1 和 2）的应用导致大血管直径减小，并通过小血管产生浓度依赖性、局部性、可逆性的血流丧失。这种血流丧失也可以被米索前列醇模拟，米索前列醇是 4 种已知 PGE 受体 EP（2-4）的激动剂，也可以被 U46619 模拟血栓烷。EP（3）和血栓烷的选择性受体拮抗剂各自部分阻断了这种反应。这是前列腺素对 CAM 血管反应性影响的首次报道。我们的模型允许独特的能力，实时和在体内检查大血管和小血管的同时反应。
