Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
Bioresour Technol. 2012 Oct;121:298-303. doi: 10.1016/j.biortech.2012.06.076. Epub 2012 Jul 3.
Microalgae have been considered as a promising feedstock for biofuels and greenhouse gas reduction. A low-cost harvesting technology without secondary contamination for down-stream extraction is a key requirement to make algal biofuel commercially viable. A novel harvesting method using ammonia as a flocculant to make the algal biomass settable was devised and studied. Another major advantage of this approach is that the ammonia added will be reused as fertilizer in the subsequent cultures. The results indicated that ammonia-induced flocculation led to more than 99% removal of algae at 12h. The OD(600) of algae growing in the ammonia-enriched flocculation medium treated with heating and CO(2) was 2 times than that of initial after 6 days. These results suggested that this flocculation method was efficient, convenient and allowed the reuse of the flocculated medium, therefore providing an option for economic harvesting and cultivation of microalgae.
微藻被认为是生物燃料和减少温室气体的有前途的原料。一种没有二次污染的低成本收获技术，用于下游提取，是使藻类生物燃料具有商业可行性的关键要求。设计并研究了一种使用氨作为絮凝剂使藻生物质可沉淀的新颖收获方法。这种方法的另一个主要优点是，添加的氨将在后一培养中再用作肥料。结果表明，在 12 小时内，氨诱导的絮凝作用可去除超过 99%的藻类。在富含氨的絮凝培养基中培养的藻类在加热和 CO(2)处理后的 OD(600)值是初始值的 2 倍，6 天后。这些结果表明，这种絮凝方法高效、方便，并且允许再利用絮凝介质，因此为经济收获和培养微藻提供了一种选择。
