Tsuboi S, Nakabayashi K, Matsumoto Y, Teno N, Tsuda Y, Okada Y, Nagamatsu Y, Yamamoto J
Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Japan.
Chem Pharm Bull (Tokyo). 1990 Sep;38(9):2369-76. doi: 10.1248/cpb.38.2369.
Various peptide fragments related to eglin c, which consists of 70 amino acid residues, were synthesized by a conventional solution method and their inhibitory effects on leukocyte elastase, cathepsin G and alpha-chymotrypsin were examined. Among them, H-Arg-Glu-Tyr-Phe-OMe (eglin c 22-25) and H-Ser-Pro-Val-Thr-Leu-Asp-Leu-Arg-Tyr-OMe (eglin c 41-49) inhibited cathepsin G and alpha-chymotrypsin but not leukocyte elastase, while H-Thr-Asn-Val-Val-OMe (eglin c 60-63) inhibited leukocyte elastase but not cathepsin G or alpha-chymotrypsin, although eglin c potently inhibited leukocyte elastase, cathepsin G and alpha-chymotrypsin. These results indicated that the interaction sites of eglin c with leukocyte elastase, cathepsin G and alpha-chymotrypsin might be different.
通过传统的溶液法合成了与由70个氨基酸残基组成的水蛭素c相关的各种肽片段，并检测了它们对白细胞弹性蛋白酶、组织蛋白酶G和α-糜蛋白酶的抑制作用。其中，H-Arg-Glu-Tyr-Phe-OMe（水蛭素c 22-25）和H-Ser-Pro-Val-Thr-Leu-Asp-Leu-Arg-Tyr-OMe（水蛭素c 41-49）抑制组织蛋白酶G和α-糜蛋白酶，但不抑制白细胞弹性蛋白酶，而H-Thr-Asn-Val-Val-OMe（水蛭素c 60-63）抑制白细胞弹性蛋白酶，但不抑制组织蛋白酶G或α-糜蛋白酶，尽管水蛭素c能有效抑制白细胞弹性蛋白酶、组织蛋白酶G和α-糜蛋白酶。这些结果表明，水蛭素c与白细胞弹性蛋白酶、组织蛋白酶G和α-糜蛋白酶的相互作用位点可能不同。
