[三七总皂苷Rg1对缺氧高碳酸血症暴露下肺动脉平滑肌细胞p38丝裂原活化蛋白激酶表达的影响]
[Effect of notoginsenoside Rg1 on p38MAPK expression of pulmonary arterial smooth muscle cells exposed to hypoxia hypercapnia].
作者信息
Tang Lan-lan, Hao Mao-lin, Li Guan-long, Wang Yuan-yuan, Zhao San, Liu Ya-kun, Wang Shu-jun, Wang Wan-tie
机构信息
Department of Pathophysiology, Wenzhou Medical College, Wenzhou, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2012 May;28(3):230-3.
OBJECTIVE
To study the effect of Notoginsenoside Rgl on p38 mitogen activated protein kinase (p38MAPK) expression in pulmonary artery smooth muscle cells (PASMCs) cultured in hypoxia hypercapnia.
METHODS
SD rat PASMCs was primary cultured, the cells of passage 2- 5 were divided into six groups: normoxic group (N group), hypoxia hypercapnia group (H group), dimethyl sulfoxide (DMSO) control group (HD group), Rg1 treated group (Rg low dose, Rg middle dose and Rg high dose group). Western blot was used to detect the expression of p-p38MAPK protein, and RT-PCR to determine the expression of p38MAPK mRNA.
RESULTS
Western blot and RT-PCR analysis indicated that the expression of p-p38MAPK protein and p-p38MAPK mRNA were significantly higher in HD group than those in N group (P < 0.01). Whereas, in Rg1 treated groups, the level of p-p38MAPK markedly decreased (P < 0.01) in dose-dependent manner.
CONCLUSION
Notoginsenoside Rg1 has protective effects on PASMCs under hypoxia hypercapnia condition, which may be related to inhibiting expression of p38MAPK.
目的
研究三七总皂苷Rg1对缺氧高碳酸培养的肺动脉平滑肌细胞（PASMCs）中p38丝裂原活化蛋白激酶（p38MAPK）表达的影响。
方法
原代培养SD大鼠PASMCs，将传代2 - 5代的细胞分为6组：常氧组（N组）、缺氧高碳酸组（H组）、二甲基亚砜（DMSO）对照组（HD组）、Rg1处理组（Rg低剂量组、Rg中剂量组和Rg高剂量组）。采用蛋白质印迹法检测p - p38MAPK蛋白表达，逆转录聚合酶链反应（RT - PCR）检测p38MAPK mRNA表达。
结果
蛋白质印迹法和RT - PCR分析表明，HD组p - p38MAPK蛋白和p - p38MAPK mRNA表达显著高于N组（P < 0.01）。而在Rg1处理组中，p - p38MAPK水平呈剂量依赖性显著降低（P < 0.01）。
结论
三七总皂苷Rg1对缺氧高碳酸条件下的PASMCs具有保护作用，其机制可能与抑制p38MAPK表达有关。
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